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Advanced Imaging Core (AIC)

The Advanced Imaging Core (AIC) provides super-resolution microscopy imaging services through its state-of-the-art equipment and expertise.

The AIC houses super-resolution microscopy imaging equipment, enabling researchers to capture sub-cellular structures and to identify protein localization patterns with high accuracy in both 2D and 3D. These cutting-edge technologies minimize sample photo damage during imaging. Live imaging on the Zeiss Elyra 7 system allows users to track fast cellular processes such as vesicle movement, and to observe a myriad of signaling events. 
In addition to maintaining the Elyra 7, the AIC offers expert technical assistance to investigators, including experimental design/consultation, microscope training, assistance with imaging, and post-acquisition data analysis. 

 

 

AIC Sim Image
Lattice SIM image acquired by Dr. Rachel Escue showing BPAECs stained with MitoTracker Red CMXRos, Alexa Fluor 488 phalloidin, and DAP.

 

 

  • The AIC houses light-sheet fluorescence, wide-field fluorescence, and super-resolution flourescence microscopy imaging equipment, enabling researchers to capture subcellular structures and to identify protein localization patterns with high accuracy in both 2D and 3D.
  • A LaVision Ultramicrscope II light-sheet fluorescent microscope (LSFM) is available in the LSFM Unit and a Zeiss Elyra 7 system is available in the super resolution microscopy (SRM) Unit.
  • These cutting-edge technologies minimize sample photodamage during imaging. Live imaging on the Elyra 7 system allows users to track fast cellular processes, such as vesicle movement, and to observe various signaling events.
  • In addition to maintaining these specialized instruments, the AIC offers expert technical assistance to investigators, including experimental design/consultation, microscope training, assistance with imaging, and post-acquisition image data analysis.

Services and Rates

 

Service Rate
Imaging with core staff assistance $87.42/hr
Imaging by trained users $26.52/hr for SIM or WF and $43.71/hr for SMLM
User Training $87.42/hr with a minimum training session of 4 hours per user.
Data analysis by core staff

$87.42/hr  (includes image pre-processing, feature registration, and feature counting) 

Workstation Use

$16.39/hr

 

Oct 31, 2024