Flow Cytometry and Cell Sorting (FCCS)
The Flow Cytometry and Cell Sorting (FCCS) Core provides state-of-the-art instruments, expertise, instruction, and assistance with experimental design and data analysis for multicolor flow cytometry and cell sorting experiments, including indexed single-cell sorting.
We offer one-on-one consultation with internal investigators at no charge for experimental design, training in the use of the instrumentation (hourly rate), software resources and data analysis (hourly rate).
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Molecular Sciences Building (858 Madison), Room 214
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Mon-Fri, 9:00am-5:00pm; after hours by appointment only
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EQUIPMENT
THE CYTEK AURORA CS
The CS spectral cell sorter uses five lasers to detect up to 64 fluorescence parameters, capturing the full fluorochrome spectrum to enable larger (40+) marker panlels and the simultaneous use of more fluorophores that would otherwise be incompatible. The five lasers include a 350nm laser (UV) with 16 detectors, 405nm laser (violet) with 16 detectors, 488nm laser (blue) with 14 detectors, 561nm laser (yellow-green) with 10 detectors and 640nm laser (red) with 8 detectors. It has three scatter detectors (FSC, SSC, 405 SSC) and 2 sets of 4 interchangeable nozzle sizes (70, 85, 100 and 130 um) for flexible, high speed sorting. The Cytek Aurora CS is capable of 2-way sorting into 15ml tubes, 4- and 6-way sorting into 1.5 and 5 mL tubes, single cell sorting into 96-well plates, and index sorting. It runs SpectroFlo software with user-friendly guided workflows. Users can create a free Cytek Cloud account to design panels and experimental templates, which can then be imported into the Cytek Aurora CS.
THE BIO-RAD ZE5 CELL ANALYZER
The ZE5 is equipped with 4 lasers capable of detecting up to 21-fluorescence parameters. The lasers include a 405nm laser with 7 fluorescence detectors, and a small particle FSC detector, a 100mW, 488 nm laser with 5 fluorescence, SSC, and FSC detectors, a 50 mW 561 nm laser with seven fluorescence detectors, and a 100 mW, 640 nm laser with three fluorescence detectors. The instrument also has the capability for small particle detection (exosomes, subcellular particles, and bacteria) and offers operator-independent programmable sample loading and data collection for up to 48 tubes and 96 well plates. The “Everest” software is more user-friendly than traditional flow cytometry software platforms.
Also available for use at no additional charge are FlowJo® and ModFit® software on workstations for advanced data analysis and cell cycle modeling.
STAFF
Elizabeth Fitzpatrick, PhD
Interim Core Director
901.448.5405
efitzpat@uthsc.edu
Dr. Fitzpatrick earned her BA in Biology from Indiana University of Pennsylvania and her PhD degree in Microbiology and Immunology from Ohio State University.
Dr. Fitzpatrick’s research focus has been understanding immunopathogenesis of Hypersensitivity pneumonitis, investigating the role of IL-17 in disease development and progression to fibrosis. Dr. Fitzpatrick has over 25 years’ experience in advanced flow cytometry techniques.
Suman Kundu, PhD
Flow Cytometry Specialist
901.448.4279
skundu1@uthsc.edu
Dr. Kundu earned his Bachelor's and Master's degrees in Microbiology and a Ph.D. in Zoology (Parasitology) from India. In 2020, Dr. Kundu joined the University of Tennessee Health Science Center as a Postdoctoral Research Fellow, focusing on innate and adaptive immune responses in spirochetal diseases while utilizing sophisticated flow cytometry techniques. Dr. Kundu has over 10 years of flow cytometry experience.