Illumina NextSeq500
| Next Seq 500 v2 kits: | NextSeq 500 High Output v2 (75 cycles; "single end" reads | NextSeq 500 High Output v2 (150 cycles; 75bp x 2 "paired end" reads) | NextSeq 500 High Output v2 (150 cycles; "single end" reads | NextSeq 500 High Output v2 (300 cycles; 150 bp x 2 "paired end" reads only) |
| Coverage | Up to 400 million reads. Best suited for sequencing DNA from one end (chIP-seq), microbiome or miRNA sequencing | Up to 400 million reads. Paired-end sequencing facilitates detection of genomic rearrangments and repetitive sequence elements, as well as gene fusions and novel transcripts. Also required for de novo sequencing. | Up to 400 million reads. Suitable for whole genome sequencing (WGS), whole transcriptome sequencing, mRNA-seq, exome sequencing | Up to 400 million reads. Paired-end sequencing facilitates detection of genomic rearrangments and repetitive sequence elements, as well as gene fusions and novel transcripts |
| Data File Size | 25-30 GB | 50-60 GB | 50-60 GB | 100-120 GB |
| Number of Samples | The number of smaples depends on coverage required. Please consult the MRC and the mBio core to design experiment | |||
| RNA-seq | 2 µg of DNase-treated RNA; low input samples contact us |
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| ChIP-seq | 1.5 ng-30 ng of ChIP DNA, low input samples (<100 ng) contact us | |||
| miRNA-seq | 2 µg of enriched miRNA or 3 µg of total RNA (contact us) | |||
| Targeted Sequencing | 200 ng-1 µg genomic input; may be possible to use the Mid Throughput v2 kits (130 million reads) contact us | |||
| Exomes | 2 µg of genomic DNA | |||
| Whole genome Sequencing | 500 ng-2 µg genomic DNA | |||
| Special Instructions |
Please consult the mBio core for experimental design and data analysis consultation; ***RNA samples must
be DNase-treated before submitting;*** |
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