Instrumentation

Orbitrap Fusion Lumos – Thermo Fisher

• Tribrid mass spectrometer combining a Quadrupole, a Dual Linear Ion Trap, and an Orbitrap mass analyzers
• Choice of Fragmentation Types: CID, HCD, ETD, ETciD, EThcD
• Excellent Mass Resolution: 15,00-450,000 (FWHM) @ m/z200
• Excellent Mass Accuracy: Sub-ppm
• Excellent Sensitivity: Quantification of 1 attomole at CV<15%
• High Scan Rate: 20Hz

UHPLC Systems:

• Ultimate 3000-RSLCnano – Thermo Fisher
• Vanquish – Thermo Fisher

Software Packages:

• Xcalibur/SII 4.3 – Thermo Fisher
• Proteome Discoverer 3.1 – Thermo Fisher
• Compound Discoverer 2.1 – Thermo Fisher
• Tracefinder 4.1 – Thermo Fisher
• Lipidsearch 4.1 – Thermo Fisher
• PMI-Preview – Protein Metrics
• PMI-Byonic - Protein Metrics
• MaxQuant – Open Source
• Skyline – Open Source