Services and Fees
UTHSC Investigators: Before preparing the samples, please read the sample preparation guidelines and requirements for an application of interest or contact the facility. Initial consultations are free.
Examples of Consultation Services Offered (Fees may apply):
- Research project design
- Sample preparation, processing recommendations, experimental design
- Letters of support, assistance in grant application
- Assistance in manuscript preparation
For Metabolomic Services:
Please contact the Biological and Small Molecule Mass Spectrometry Core (BSMMSC) at UT Knoxville.
Fee Structure for UTHSC Internal and External Users
- UTHSC Internal Users – Please use fee schedule for services listed below to calculate total service cost
- External Users - Please use fee schedule for services listed below to calculate total service cost and then add 50%
| Services | Service Code | Unit | $$/unit (FY24-25) |
| Agilent QC test - up to 10 amples per chip | 100 | per chip | 61.50 |
| In-gel trypsin digestion | 101 | per band | 49.19 |
| In-solution trypsin digestion | 102 | per sample | 49.19 |
| In-solution LysC/trypsin digestion | 102a | per sample | 73.80 |
| Reduction/alkylation | 103 | per sample | 24.62 |
| Desalting of peptide mixtures - ZipTip | 104 | per sample | 12.31 |
| Detergent removal/clean-up through acetone precipitation of proteins | 105 | per sample | 24.62 |
| Protein concentration - BCA assay in triplicates - at least 3 samples | 106 | per sample | 24.62 |
| Peptide (mixture) concentration - BCA assay in triplicates - at least 3 samples | 107 | per sample | 24.62 |
| High pH Reversed-Phase (HpHRP) Fractionation - 8 step fractions | 110 | per sample |
196.76 |
| Complete sample processing starting from cleared cell lysate, membrane pellets, or cell pellets | |||
| Processing for following LC/MS/MS analysis | 111 | set-up | 245.98 |
| 111a | per sample | 61.50 | |
| Processing for following label-free differential protein expression analysis | 112 | set-up | 245.98 |
| 112a | per sample | 61.50 | |
| Processing for following TMT/iTRAQ labeling | 113 | set-up | 245.98 |
| 113a | per sample | 61.50 | |
| TMT/iTRAQ labeling/preparation for LC and MS analysis, 4-plex (set of 4 samples) | 114 | per set | 860.96 |
| TMT/iTRAQ labeling/preparation for LC and MS analysis, 6-plex (set of 6 samples) | 116 | per set | 1,229.88 |
| TMT/iTRAQ labeling/preparation for LC and MS analysis, 8-plex (set of 8 samples) | 118 | per set | 1,537.35 |
| TMT/iTRAQ labeling/preparation for LC and MS analysis, 10-plex (set of 10 samples) | 119 | per set | 1,660.34 |
| TMT/iTRAQ labeling, 16-plex (set of 16 samples) | 120 | per set | 1,744.54 |
| TMT/iTRAQ labeling, 16-plex (set of 16 samples) | 120a | per set | 2,021.55 |
| TMT/iTRAQ labeling, 18-plex (set of 18 samples) | 120b | per set | 2,200.00 |
| TiO2 enrichment of phospho-peptides | 121a | per run | 196.97 |
| Fe-NTA enrichment of phospho-peptides | 121b | per run | 196.97 |
| SMOAC (TiO2+Fe-NTA) enrichment of phospho-peptides | 121c | per run | 337.65 |
| Services | Service Code | Unit | $$/Unit (FY24-25) |
| Determination of the Molecular Mass: More Information here. | |||
| Infusion ESI-HRAM MS analysis - small analyte | 201 | per sample | 59.71 |
| Intact protein | 202 | per sample | 95.52 |
| Service | Service Code | Unit |
$$/Unit (FY24-25) |
|
Protein Identification: More Information here. LC/MS/MS, DB search (does not include sample digestin/preparation) |
|||
| RP LC/MS/MS - 30 min gradient | 300 | per sample | 86.08 |
| RP LC/MS/MS - 60 min gradient | 301 | per sample | 135.29 |
| RP LC/MS/MS - 120 min gradient | 302 | per sample | 221.38 |
| RP LC/MS/MS - 240 min gradient | 304 | per sample | 368.96 |
| MudPIT, DB search (does not include sample digestin/preparation) | |||
| 6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 310 | per fraction | 172.20 |
| 7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 311 | per fraction | 166.05 |
| >12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 312 | per fraction | 159.89 |
|
Protein Identification and Mapping of Specified PTMs: More Information here. LC/MS/MS, DB/PTM search (does not include sample digestin/preparation) |
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| RP LC/MS/MS - 60 min gradient | 401 | per sample | 135.29 |
| RP LC/MS/MS - 120 min gradient | 402 | per sample | 221.38 |
| RP LC/MS/MS - 240 min gradient | 403 | per sample | 368.96 |
| Search for specified PTMs | 404 | per PTM | 36.88 |
|
Differential Protein Expression Analysis - iTRAQ/TMT Based Analysis (Reporter Ion quantification): More Information here. |
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|
LC/MS/MS, DB search/quantification (does not include sample labeling/preparation) |
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| Unfractionated mixture of labeled peptides - 2 hr RP-LC/MS/MS | 500 | per run | 245.98 |
| Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS | 500a | per run | 405.86 |
|
MudPIT, DB search/quantification (does not include sample labeling/preparation) |
|||
| 6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 501 | per fraction | 184.48 |
| 7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 502 | per fraction | 178.32 |
| >12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 503 | per fraction | 172.20 |
|
Differential Protein Expression Analysis - SILAC Analysis (Precursor Ion Quantification): More Information here. |
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|
LC/MS/MS, DB search/quantification (does not include sample labeling/preparation) |
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| Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS | 510 | per run | 245.98 |
| Unfractionated mixture of labeled peptides - 4 hr RP-LC/MS/MS | 510a | per run | 405.86 |
|
MudPIT, DB search/quantification (does not include sample labeling/preparation) |
|||
| 6 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 511 | per fraction | 184.48 |
| 7-12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 512 | per fraction | 178.32 |
| >12 (SCX or HpHRP) step fractions - 2 hr RP-LC/MS/MS per fraction | 513 | per fraction | 172.20 |
|
Differential Protein Expression Analysis - Label Free Analysis (Precursor ion Peak Area Detection): More Information here. RP-LC/HRAM-MS/MS, post-acquisition analysis (does not include sample digestion/preparation) |
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| RP-LC/HRAM-MS/MS - 60 min gradient | 520 | per run | 159.88 |
| RP-LC/HRAM-MS/MS - 120 min gradient | 521 | per run | 245.98 |
| RP-LC/HRAM-MS/MS - 240 min gradient | 522 | per run | 405.86 |
|
Absolute Quantification of Specified (Target) Proteins - Parallel Reaction Monitoring (PRM) PRM analysis for peptide quantification (does not include sample digestin/preparation) RP LC/MS/MS, post-acquisition analysis, Peptide Internal Standard, AUC quantification |
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| RP LC/MS/MS - peptide quantification, 30 min gradient | 531 | per run | 98.39 |
| RP LC/MS/MS - peptide quantification, 60 min gradient | 532 | per run | 153.74 |
| RP LC/MS/MS - peptide quantification, 120 min gradient | 533 | per run | 245.98 |